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Toxicity assessment and bioaccumulation in zebrafish embryos exposed to carbon nanotubes suspended in Pluronic® F-108.

TitleToxicity assessment and bioaccumulation in zebrafish embryos exposed to carbon nanotubes suspended in Pluronic® F-108.
Publication TypeJournal Article
Year of Publication2016
AuthorsWang R, Meredith AN, Lee M, Deutsch D, Miadzvedskaya L, Braun E, Pantano P, Harper S, Draper R
JournalNanotoxicology
Volume10
Issue6
Pagination689-98
Date Published2016 Aug
ISSN1743-5404
Abstract

Carbon nanotubes (CNTs) are often suspended in Pluronic® surfactants by sonication, which may confound toxicity studies because sonication of surfactants can create degradation products that are toxic to mammalian cells. Here, we present a toxicity assessment of Pluronic® F-108 with and without suspended CNTs using embryonic zebrafish as an in vivo model. Pluronic® sonolytic degradation products were toxic to zebrafish embryos just as they were to mammalian cells. When the toxic Pluronic® fragments were removed, there was little effect of pristine multi-walled CNTs (pMWNTs), carboxylated MWNTs (cMWNTs) or pristine single-walled carbon nanotubes (pSWNTs) on embryo viability and development, even at high concentrations. A gel electrophoretic method coupled with Raman imaging was developed to measure the bioaccumulation of CNTs by zebrafish embryos, and dose-dependent uptake of CNTs was observed. These data indicate that embryos accumulate pMWNTs, cMWNTs and pSWNTs yet there is very little embryo toxicity.

DOI10.3109/17435390.2015.1107147
Alternate JournalNanotoxicology
PubMed ID26559437
PubMed Central IDPMC4864110
Grant ListR01 ES017552 / ES / NIEHS NIH HHS / United States
R15 CA152917 / CA / NCI NIH HHS / United States
R15 ES023666 / ES / NIEHS NIH HHS / United States
Project Reference: 
Zebrafish EZ Metric Assay

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